rabbit anti gdf9  (Cusabio)

Journal: Heliyon

Article Title: Comparing GDF9 in mature follicles and clinical outcomes across different PCOS phenotype

doi: 10.1016/j.heliyon.2024.e29879

Figure Lengend Snippet: Distribution of GDF9 in FF from the three PCOS phenotypes and control group. Data are presented as mean ± SD. d P < 0.01, compared with Control Group; a P < 0.01, compared with Control Group; b P < 0.05, compared with PCOS A; c P < 0.05, compared with PCOS B.

Article Snippet: Briefly, the CCs cell slices were fixed with 4 % paraformaldehyde in phosphate-buffered saline for 15 min, then immersed in 3 % hydrogen peroxide to block endogenous peroxidase activity, and subsequently blocked in goat serum for 1 h. The slices were then incubated overnight at 4 °C with primary antibodies rabbit anti-GDF9 (GDF9, Abcam, USA, 1:100), followed by incubation with biotin-labeled anti-rabbit secondary antibody for half an hour.

Techniques:

Journal: Heliyon

Article Title: Comparing GDF9 in mature follicles and clinical outcomes across different PCOS phenotype

doi: 10.1016/j.heliyon.2024.e29879

Figure Lengend Snippet: Expression of GDF9 in the CCs. (a) Immunohistochemical staining of GDF9 expressed in the control group, phenotype A, phenotype B and phenotype D. (Original magnification: × 400, bars, 50 μm). (b) Mean density of GDF9 in CCs of the three PCOS phenotypes and control group, a P < 0.01, compared with Control Group; b P < 0.01, compared with PCOS D; c P < 0.01, compared with Control Group; d P < 0.01, compared with PCOS D.

Article Snippet: Briefly, the CCs cell slices were fixed with 4 % paraformaldehyde in phosphate-buffered saline for 15 min, then immersed in 3 % hydrogen peroxide to block endogenous peroxidase activity, and subsequently blocked in goat serum for 1 h. The slices were then incubated overnight at 4 °C with primary antibodies rabbit anti-GDF9 (GDF9, Abcam, USA, 1:100), followed by incubation with biotin-labeled anti-rabbit secondary antibody for half an hour.

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Heliyon

Article Title: Comparing GDF9 in mature follicles and clinical outcomes across different PCOS phenotype

doi: 10.1016/j.heliyon.2024.e29879

Figure Lengend Snippet: Analysis of multiple factors affecting blastocyst formation and clinical pregnancy rate in the PCOS phenotypes.

Article Snippet: Briefly, the CCs cell slices were fixed with 4 % paraformaldehyde in phosphate-buffered saline for 15 min, then immersed in 3 % hydrogen peroxide to block endogenous peroxidase activity, and subsequently blocked in goat serum for 1 h. The slices were then incubated overnight at 4 °C with primary antibodies rabbit anti-GDF9 (GDF9, Abcam, USA, 1:100), followed by incubation with biotin-labeled anti-rabbit secondary antibody for half an hour.

Techniques:

Journal: Theranostics

Article Title: Polycomb repressive complex 1 modulates granulosa cell proliferation in early folliculogenesis to support female reproduction

doi: 10.7150/thno.89878

Figure Lengend Snippet: Crosstalk in dcKO was disrupted with impaired physical connections and signaling pathways. ( A ) Schematic diagram and ( B ) strategy of LCM assays. OO, oocyte. ( C ) Key GO enrichment of all DEGs in GCs. ( D ) TOP15 terms related to ion channels of downregulated DEGs in GCs. ( E ) GSEA of all DEGs in GCs. NES, normalized enrichment score. ( F ) CX43 staining (green) in 3-week-old ovaries. Dotted rectangles mark the areas that are enlarged on the right. ( G ) Phalloidin staining (red) in 3-week-old ovaries. Dotted rectangles mark the areas that are enlarged on the right, which are inverted to black/white to highlight OO-Mv. ( H ) TZPs (dotted lines) in 3-week-old ovaries. ZP, zona pellucida. ( I ) GDF9, SMAD3 and p-SMAD3 protein levels in 3-week-old ovaries. ( J ) RDX staining (green) in 3-week-old ovaries. Arrowheads mark the OO-Mv in the WT group. ( K ) Density of OO-Mv per follicle. n = 8 (WT), n = 6 (dcKO). ( L ) SMAD3 staining (green) in 3-week-old ovaries. Dotted rectangles mark the areas are enlarged on the right. Dotted cycle on the right marks the nucleus of a single GC.

Article Snippet: Primary antibodies and dilution rates were as follows: rabbit anti-α-Tubulin (1:1000, Beyotime biotechnology, Cat#AF0001); rabbit anti-H3 (1:1000, Abcam, Cat#ab1791); rabbit anti-GAPDH (1:1000, Proteintech, Cat#10494); rabbit anti-DDX4 (1:1000, Abcam, Cat#13840); goat anti-FOXL2 (1:500, Novus, Cat#NB100-1277); rabbit anti-BMI1 (1:500, Cell Signaling Technology, Cat#6964); rabbit anti-MEL18 (1:1000, provided by Prof. Qun He); rabbit anti-H2AK119ub1 (1:500, Cell Signaling Technology, Cat#8240); rabbit anti-GDF9 (1:500, Abcam, Cat#ab38544); rabbit anti-SMAD3 (1:500, Beyotime biotechnology, Cat#AF1501); rabbit anti-p-SMAD3 (1:500, Cell Signaling Technology, Cat#C25A9); mouse anti-p-RB (1:500, Santa Cruz, Cat#377528); mouse anti-E2F1 (1:500, Santa Cruz, Cat#251); rabbit anti-PCNA (1:500, Beyotime biotechnology, Cat#AF1363); rabbit anti-Caspase-3 (1:500, Cell Signaling Technology, Cat#9662).

Techniques: Staining

Journal: Antioxidants

Article Title: Tannin Reduces the Incidence of Polyspermic Penetration in Porcine Oocytes

doi: 10.3390/antiox11102027

Figure Lengend Snippet: Detection of GDF9 and BMP15 proteins by immunofluorescence staining and detection of p-CKD1 and CKD1 proteins by Western blot in oocytes. ( A , B ) GDF9 protein expression. ( C , D ) BMP15 protein expression. ( E ) p-CDK1 and CDK1 protein expression. Within the same indicator, bars with different letters indicate a significant difference ( p < 0.05). Results are shown as the average ± SEM of at least three repeats of independent experiments. A total of 30 oocytes were analyzed per group in each replicate.

Article Snippet: Porcine oocytes were incubated with rabbit polyclonal antibodies against GDF9 (ab93892; Abcam, Cambridge, UK) and BMP15 (PA5-34401; Invitrogen) at 37 °C for 2 h. The samples were washed three times in 2% BSA and then incubated with a goat anti-rabbit fluorescein isothiocyanate-conjugated secondary antibody (1:200; Jackson Immuno Research Laboratories Inc., West Grove, PA, USA) for 2 h. Oocytes were mounted on glass slides and the images were captured using an epifluorescence microscope (TE2000-S; Nikon).

Techniques: Immunofluorescence, Staining, Western Blot, Expressing

Journal: Cell Death & Disease

Article Title: CPEB3 deficiency in mice affect ovarian follicle development and causes premature ovarian insufficiency

doi: 10.1038/s41419-021-04374-4

Figure Lengend Snippet: A Western blot analysis of GAPDH, GDF9, PCNA, and Cleaved-Caspase3 protein expression in 3-month-old WT and Cpeb3 -mutant ovaries. GAPDH was used as the internal control. B Western blot analysis of GDF9 and GAPDH in WT and Cpeb3 -mutant oocytes. GAPDH was used as the internal control. C Ovarian sections stained for GDF9 at 3-month-old WT and Cpeb3 -mutant ovaries. D Gdf9 mRNA levels were performed by qRT-PCR in WT and Cpeb3- mutant oocytes. Gapdh was used as the internal control. Scale bar = 50 μm and 25 μm in C . *** P < 0.001.

Article Snippet: Rabbit polyclonal anti-GDF9 antibody was purchased from Abcam (ab93892).

Techniques: Western Blot, Expressing, Mutagenesis, Control, Staining, Quantitative RT-PCR